刘家源;刘文科;
LIU Jiayuan;LIU Wenke;Key Laboratory of Energy Conservation and Waste Management of Agricultural Structures, Ministry of Agriculture and Rural Affairs,Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences;

• 1:中国农业科学院农业环境与可持续发展研究所农业农村部设施农业节能与废弃物处理重点实验室

摘要(Abstract)：

为了探明采前LED红蓝光连续光照光强对三种氮水平水培生菜(Lactuca sativa)AsA代谢的影响,在密闭植物工厂内,以红蓝LED(红光∶蓝光=4∶1)为光源,设置2种光强(100、150μmol·m^(-2)·s(-2)·s^(-1))和3种氮水平(2.5、5、7.5 mmol/L)进行水培生菜培养,在采收前第3天(定植第18天)开始进行为期3天的连续光照处理(150μmol·m(-1))和3种氮水平(2.5、5、7.5 mmol/L)进行水培生菜培养,在采收前第3天(定植第18天)开始进行为期3天的连续光照处理(150μmol·m^(-2)·s(-2)·s^(-1),红光∶蓝光=4∶1)。研究了氮水平与光强对生菜AsA含量和AsA合成代谢关键酶活性的影响。结果表明,提高氮水平和光强可以提高水培生菜的AsA含量。随着氮水平的上升,DHA含量呈下降趋势。连续光照前,AsA含量的增加是由于氮水平与光强的升高提高了参与AsA再生循环系统的MDHAR酶活性。连续光照后,低氮水平低光强下生菜的AsA含量增幅相比高氮水平高光强水培生菜更高。总之,AsA含量增加是因为供氮条件与连续光照耦合处理提高了GR和DHAR的酶活性,从而促进了AsA的累积。
In order to find out the effect of LED red and blue continuous light intensity on AsA metabolism of hydroponic lettuce with three nitrogen levels before harvest, red and blue LEDs(red∶blue=4∶1)were used in a closed plant factory. Light source with 2 kinds of light intensity(100, 150 μmol·m(-1),红光∶蓝光=4∶1)。研究了氮水平与光强对生菜AsA含量和AsA合成代谢关键酶活性的影响。结果表明,提高氮水平和光强可以提高水培生菜的AsA含量。随着氮水平的上升,DHA含量呈下降趋势。连续光照前,AsA含量的增加是由于氮水平与光强的升高提高了参与AsA再生循环系统的MDHAR酶活性。连续光照后,低氮水平低光强下生菜的AsA含量增幅相比高氮水平高光强水培生菜更高。总之,AsA含量增加是因为供氮条件与连续光照耦合处理提高了GR和DHAR的酶活性,从而促进了AsA的累积。
In order to find out the effect of LED red and blue continuous light intensity on AsA metabolism of hydroponic lettuce with three nitrogen levels before harvest, red and blue LEDs(red∶blue=4∶1)were used in a closed plant factory. Light source with 2 kinds of light intensity(100, 150 μmol·m^(-2)·s(-2)·s^(-1))and 3 kinds of nitrogen levels(2.5, 5, 7.5 mmol/L)for hydroponic lettuce cultivation, 3 days before harvest(colonization on the 18 th day)a 3-day continuous light treatment(150 μmol·m(-1))and 3 kinds of nitrogen levels(2.5, 5, 7.5 mmol/L)for hydroponic lettuce cultivation, 3 days before harvest(colonization on the 18 th day)a 3-day continuous light treatment(150 μmol·m^(-2)·s(-2)·s^(-1), red∶blue=4∶1)was started. The effects of nitrogen level and light intensity on the AsA content and AsA anabolism key enzyme activities of lettuce were studied. The results show that increasing the nitrogen level and light intensity can increase the AsA content of hydroponic lettuce. As the nitrogen level increases, the DHA content shows a downward trend. Before continuous light, the increase in AsA content was due to the increase in nitrogen level and light intensity, which increased the MDHAR enzyme activity involved in the AsA regeneration cycle. After continuous light, the AsA content of lettuce under low nitrogen level and low light intensity increased more than that of hydroponic lettuce with high nitrogen level and high light intensity. In short, the increase in AsA content is because the nitrogen level coupled with continuous light treatment increases the enzymatic activity of GR and DHAR, thereby promoting the accumulation of AsA.

关键词(KeyWords)： 光强;连续光照;氮水平;AsA;代谢酶
light intensity;continuous illumination;nitrogen level;AsA;metabolic enzyme

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金面上项目(31672202);; “十三五”国家重点研发计划项目(2016YFD0801001)

作者(Authors): 刘家源;刘文科;
LIU Jiayuan;LIU Wenke;Key Laboratory of Energy Conservation and Waste Management of Agricultural Structures, Ministry of Agriculture and Rural Affairs,Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences;

参考文献(References)：

• [1] 王晓琳.氮素水平对烟草幼苗叶片光合特性的影响[D].北京：中国农业科学院，2019.
• [2] 张玉彬，查凌雁，刘文科，等.LED红蓝光采前连续光照对不同氮水平与光强下水培生菜产量及品质的影响[C]//2019年中国照明论坛——半导体照明创新应用暨智慧照明发展论坛论文集.北京：中国照明学会，2019.
• [3] 周恺.植物工厂条件下氮素与光照互作对雾培生菜产量和抗氧化营养品质的影响[D].杭州：浙江大学，2016.
• [4] 安华明，陈力耕，樊卫国，等.刺梨果实中维生素C积累与相关酶活性的关系[J].植物生理与分子生物学学报，2005(4):431-436.
• [5] Kotchoni S O,Larrimore K E,Mukherjee M,et al.Alterations in the endogenous ascorbic acid content affect flowering time in Arabidopsis[J].Plant Physiology,2009,149(2):803-815.
• [6] Yabuta Y,Maruta T,Nakamura A.Conversion of L-galactono-1,4-lactone to L-ascorbate is regulated by the photosynthetic electron transport chain in Arabidopsis[J].Bioscience,Biotechnology,and Biochemistry,2008,72(10):2598-2607.
• [7] Bartoli C G,YU J,Gómez F,et al.Inter-relationships between light and respiration in the control of ascorbic acid synthesis and accumulation in Arabidopsis thaliana leaves[J].Journal of Experimental Botany,2006,57(8):1621-1631.
• [8] Cakmak I,Marschner H.Magnesium deficiency and high light intensity enhance activities of superoxide dismutase,ascorbate peroxidase,and glutathione reductase in bean leaves[J].Plant Physiology,1992.98(4):1222-1227.
• [9] Smirnoff N.Ascorbate biosynthesis and function in photoprotection[J].Philosophical Transactions of the Royal Society B:Biological Sciences,2000,355(1402):1455-1464.
• [10] Velez-ramirez A I,Ieperen W V,Vreugdenhil D,et al,Plants under continuous light[J].Trends in Plant Science,2011,16(6):310-318.
• [11] 陈文昊，徐志刚，刘晓英，等.LED光源对不同品种生菜生长和品质的影响[J].西北植物学报，2011,31(7):1434-1440.
• [12] 周晚来，采收前短期连续光照降低水培生菜硝酸盐含量的效果研究[D].北京：中国农业科学院，2011.
• [13] 周华，刘淑娟，王碧琴.不同波长LED光源对生菜生长和品质的影响[J].江苏农业学报，2015,31(2):429-433.
• [14] Gillespie K M,Ainsworth E A.Measurement of reduced,oxidized and total ascorbate content in plants[J].Nature Protocols,2007,2(4):871-874.
• [15] Li M,Liang D,Pu F,et al.Ascorbate levels and the activity of key enzymes in ascorbate biosynthesis and recycling in the leaves of 22 Chinese persimmon cultivars[J].Scientia Horticulturae,2009,120(2):250-256.
• [16] Nakano Y,Asada K.Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts[J].Plant and Cell Physiology,1981,22(5):867-880.
• [17] Ma F,Cheng L.Exposure of the shaded side of apple fruit to full sun leads to up-regulation of both the xanthophyll cycle and the ascorbate-glutathione cycle[J].Plant Science,2004,166(6):1479-1486.
• [18] 余意，杨其长，刘文科.LED短期连续光照与氮营养对水培生菜品质的影响[J].应用生态学报，2015,26(11):3361-3366.
• [19] Zhou W L,Liu W K,Yang Q C.Reducing nitrate content in lettuce by pre-harvest continuous light delivered by red and blue light-emitting diodes[J].Journal of Plant Nutrition,2013,36:481-490.
• [20] Tamaoki M,Mukai F,Asai N,et al,Light-controlled expression of a gene encoding Lgalactono-g-lactone dehydrogenase which affects ascorbate pool size in Arabidopsis thaliana[J].Plant Science,2003,164:1111-1117.
• [21] Loscos J,Matamoros M A,Becana M.Ascorbate and homoglutathione metabolism in common bean nodules under stress conditions and during natural senescence[J].Plant Physiology,2008,146(3):1282-1292.
• [22] Gallie D R.The role of l-ascorbic acid recycling in responding to environmental stressand in promoting plant growth[J].Journal of Experimental Botany,2013(2):433-443.
• [23] Zhang L,Ma G,Yamawaki K,et al.Regulation of ascorbic acid metabolism by blue LED light irradiation in citrus juice sacs[J].Plant Science,2015,233:134-142.